Lasers & Optical Fibers Technologies
RMC1 Re-scan Confocal Microscope VIS or NIR / Microscopes imaging
The RCM1 is a highly sensitive super-resolution confocal system. It’s capable of reaching a 120nm resolution after deconvolution (170nm raw). Study living cells in high detail with minimal phototoxicity and photobleaching.
RMC1 Re-scan Confocal Microscope VIS or NIR summary
RCM1 is based on the Re-scan Confocal Microscope (RCM) principle. Capture datasets with 170nm raw resolution (120nm after deconvolution) by using 60x to 100x high numerical aperture (NA) magnification objectives and keep the laser intensity at a minimum. RCM1 acquires super-resolution images with only 85 nano-watts laser power.
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Features
- Super-resolution confocal system
- High sensitivity
- Excellent add-on to any widefield microscope
- Easy to use
- API integration into third-party software
- VIS or NIR versions available
Applications
- Live cell dynamics
- Mitochondria dynamics and structure analysis
Specifications
| RCM1 | |
|---|---|
| Detector | Camera |
| Resolution | 120nm (after deconvolution, raw image = 170nm) |
| Sensitivity | Up to 95% QE |
| FOV | 130×130µm (60x, super-resolution) |
| Optimized for | 100x, 60x (high NA) |
| Scanner | Analog (open-loop) |
| Speed | 1fps @ 512x512 pixels |
| Wavelength | VIS or NIR |
| Software | Micromanager, NIS Elements |
| Integration | API |
| PSF for deconvolution with | Microvolution, SVI Huygens |
| Bypass mode | Yes |
High contrast imaging
Hela cell stained for DNA (blue), Actin (green), Tubulin (red) and clathrin (yellow). Captured on RCM1 using a 100x 1.45 objective. Sample courtesy: Nicolas Touret, University of Alberta, Canada.
